The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. This product is related to the following categories: Restriction Endonucleases: N-O, Time-Saver Qualified Restriction Enzymes Products This product can be used in the following applications: Restriction Enzyme Digestion coli strain that carries the cloned NotI gene from Nocardia otitidis-caviarum (ATCC 14630). NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying. All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). We are excited to announce that all reaction buffers are now BSA-free. Price (£) R3192L: 5000 UNITS: 20,000 units/ml: £243.00: R3192S: 1000 UNITS: 20,000 units/ml: £60.00: High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer). Time-saver qualified enzymes digest 1 g of substrate DNA in just 5-15 minutes using 1 l of enzyme under. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. For results in just 5-15 minutes, choose one of the 190 time-saver qualified enzymes from NEB. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.įor details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.Īn E. XbaI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10128088. Typically, a restriction digest means incubating 1 l of enzyme with 1 g of purified DNA in a final volume of 50 l for 1 hour. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Expected Bands NotI-HF/ PmeI: 8.8 kb & 4.2 kb. This gel depicts the NEB 1kb ladder and a digestion of the plasmid iCas with the following enzymes: NotI-HF and PmeI. We predict the following banding patterns: NotI-HF single. HF enzymes also exhibit dramatically reduced star activity. This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 84232 with NotI-HF and XbaI. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing.

NotI has a High Fidelity version NotI-HF ® ( NEB #R3189).

Neb noti hf